Application

Research CategorySignaling

Research Sub CategoryDevelopmental Signaling

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected GPAT-1 in rat brain tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected GPAT-1 in rat brain cells.

Anti-GPAT-1 Antibody is an antibody against GPAT-1 for use in western blotting, IHC & immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAT-1) is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT-1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT-1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT-1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT-1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets.

Immunogen

Epitope: C-terminus

KLH-conjugated synthetic peptide corresponding to the C-terminus of human GPAT-1.

Linkage

Replaces: MABS402

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected GPAT-1 in rat brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~82 kDa observed. Uncharacterized bands may be observed in some lysate(s).