Application

Research Sub CategorySignaling Neuroscience

Research CategoryNeuroscience

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected GLUT9 in human cerebellum and hindbrain tissues.

Detect GLUT9 using this Anti-GLUT9 Antibody validated for use in western blotting & IHC (Paraffin).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GLUT9, also known as Glucose transporter type 9 or GLUT-9, and encoded by the gene SLC2A9/GLUT9, is a member of the sugar transport family that primarily transports urate and fructose; GLUT9 does also transport glucose but at a lower rate than its preferred sugars. GLUT9 also plays a role in urate reabsorption by the proximal tubules of the kidney. GLUT9 is localized to the plasma membrane. GLUT9 is most strongly expressed in basolateral membranes of proximal renal tubular cells, liver and placenta. GLUT9 is also detected in lung, blood leukocytes, heart skeletal muscle and chondrocytes from articular cartilage. Isoform 2 is only detected in the apical membranes of polarized renal tubular cells and placenta. Defects in GLUT9 may be associated with Hypouricemia renal 2 (RHUC2) a disorder characterized by impaired uric acid reabsorption at the apical membrane of proximal renal tubule cells, and high urinary urate excretion.

Immunogen

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human GLUT9.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected GLUT9 in 10 µg of human liver tissue lysate.

Specificity

This antibody is expected to recognize both known isoforms of GLUT9.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~62 kDa observed. Uniprot describes 2 isoforms at 59 kDa (isoform 1) and 55 kDa (isoform 2).