Anti-GITRL

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Detect GITRL using this Anti-GITRL Antibody validated for use in western blotting & ICC.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected GITRL in THP-1 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tumor necrosis factor ligand superfamily member 18, also known as Activation-inducible TNF-related ligand (AITRL) or Glucocorticoid-induced TNF-related ligand (hGITRL) and encoded by the gene name TNFSF18 or AITRL or GITRL or TL6, regulates numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-κB and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions. Its ligand GITRL is expressed in endothelial and antigen-presenting cells (4) and can activate NF-κB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR. Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-κB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.

Immunogen

KLH-conjugated recombinant human GITRL ligand.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in recombinant GITRL protein.

Western Blotting Analysis: 1 µg/mL of this antibody was detected in recombinant GITRL protein.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~26 kDa observed. Uncharacterized bands may be observed in some lysate(s).