Application

Research Sub CategoryImmunological Signaling

Research CategorySignaling

Western Blotting Analysis: A representative lot from an independent laboratory detected GBP5 in differentiating THP-1 cells which were untreated or treated with LPS alone, or LPS/IFN-gamma (Shenoy, A. R., et al. (2012). Science. 336(6080):481-485.).

Detect GTP binding protein using this rabbit polyclonal antibody, Anti-GBP5 Antibody validated for use in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GBP5, also known as GBP-TA antigen, or GTP-binding protein 5, or Guanine nucleotide-binding protein 5 or GBP-5, and encoded by the gene GBP5, is a protein that plays a role in the inflammatory response to pathogens and may also play a role in apoptosis. GBP5 stimulates cryopryrin (NLRP3 protein) assembly of the intracellular inflammasome, a multiprotein oligomer consisting of caspase 1, PYCARD, NALP and sometimes caspase 5. Inflammasomes act as sensory complexes that alert the immune system to the presence of infection or tissue damage. Nucleosomes and GBP5 are expressed in myeloid cells and are a component of the innate immune system; GBP5 acts as key rheostat for activation and assembly of the inflammasomes. Additionally, GBP5 is a GTPase that homodimerizes upon GTP-binding; dimerization is required for the second hydrolysis step from GDP to GMP. GBP5 is localized to the cytoplasm and intracellular membranes via its lipid-anchor sequences.

Immunogen

Linear peptide corresponding to human GBP5.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected GBP5 in 10 µg of K562 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~67 kDa observed. A second isoform is known to exist at 55 kDa Uncharacterized band(s) may be observed in some cell lysates.