Application

Research Sub CategoryNuclear Receptors

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected GABPB (NRF-2beta) occupancy at the TFB1M and TFB2M promoters by ChIP using human embryonic kidney 293FT chromatin preparations (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of GABP A2/B2 (NRF-2 α2/β2) heterotetramer-DNA complex in EMSA using in vitro translated GABP (NRF-2) subunits and radiolabeled cytochrome oxidase subunit IV promoter fragment containing tandem GABP (NRF-2) recognition sites (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GABPB (NRF-2beta)-PRC complex from human embryonic kidney 293FT cell lysate (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
Western Blotting Analysis: A representative lot detected GABPB (NRF-2beta) in U2OS cell lysates (Gleyzer, N., and Scarpulla, R.C. (2011). J. Biol. Chem. 286(46):39715-39725).

This Anti-GABPB-1/NRF2b Antibody is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility Shift Assay for the detection of GABPB-1/NRF2b.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GA-binding protein subunit beta-1 (EC 3.2.1.3; UniProt Q06547; also known as GABP subunit beta-1, GABP subunit beta-2, GABPB-1, GABPB-2, Nuclear respiratory factor 2, Transcription factor E4TF1-47, Transcription factor E4TF1-53) is encoded by the GABPB1 (also known as E4TF1, E4TF1B, GABPB, GABPB2, NRF2B1, NRF2B2) gene (Gene ID 2553) in human. Initially identified through their interactions with cytochrome C and cytochrome oxidase promoters, GABP (NRF-2) and the related nuclear respiratory factor NRF-1 target many nuclear genes essential for mitochondrial respiratory function, including the mitochondrial transcription specificity factors TFB1M and TFB2M. GABP plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. GABP is a tetrameric complex composed of the NRF-2alpha (GABPA) and the NRF-2beta (GABPB) subunits. GABPA binds DNA via its ETS domain, and it recruits GABPB, which activates transcription through a glutamine-rich region in its carboxy terminus. GABPB and PRC, a member of PGC-1 family of regulated coactivators, can both interact with host cell factor 1 (HCF-1). PRC can therefore associate with GABP indirectly via HCF-1 and participate in GABP-dependent expression of the mitochondrial transcription factors TFB1M and TFB2M.

Immunogen

Recombinant protein corresponding to human GABPB-1/NRF2b.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected GABPB-1/NRF2b in 10 µg of HeLa cell lysate.

Specificity

Expected to react with all 4 spliced variants: isoform 1 (GABPB-1, Beta-1), isoform 2 (Beta-2), isoform 3 (GABPB-2, Gamma-1), isoform 4 (Gamma-2)

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~48 kDa observed