Application

Research CategoryApoptosis & Cancer

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected FAM60A in 10 µg of A549 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FAM60A in human prostate cancer and human breast cancer.

Research Sub CategoryApoptosis - Additional

Anti-FAM60A Antibody detects level of FAM60A & has been published & validated for use in FAM60A.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

FAM60A, also known as Protein FAM60A, or Tera protein homolog, and encoded by the gene FAM60A/C12orf14, is a subunit of the Sin3 deacetylase complex (Sin3/HDAC) that is important for the repression of genes. The SIN3A-HDAC complex deacetylates histones thereby repressing gene transcription. FAM60A specifically impacts genes encoding components of the TGF-beta signaling pathway and promoters of important proteins like cyclin D1. FAM60A activity peaks during G1 and S phases of the cell cycle and is up regulated in many carcinoma and tumor tissues. Additionally, loss of FAM60A leads to a change in cell morphology, an increase in cell migration, increased histone acetylation at the cyclin D1 promoter and elevated levels of cyclin D1 mRNA and protein. Furthermore, depletion of FAM60A altered the periodic association of HDAC1 with the cyclin D1 promoter, increased cyclin D1 expression at all cell cycle phases, and caused premature S phase entry.

Immunogen

KLH-conjugated linear peptide corresponding to human FAM60A.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in transfected FAM60A cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected FAM60A in 10 µg of transfected FAM60A cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~35 kDa observed. Although endogenous expected and detected ~ 25kDa , transfected seen at ~35kDa