Analysis Note

ControlHEK293 cell lysate

Application

Immunocytochemistry Analysis: A 1:500 dilutioin from a previous lot detected WASH complex subunit FAM21C in NIH/3T3, A431, and HeLa cells.

Anti-WASH complex subunit FAM21C Antibody detects level of WASH complex subunit FAM21C & has been published & validated for use in WB & IC.

Research CategoryCell Structure

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

FAM21C, also known as WASH complex subunit FAM21C, is a part of the a 500 kDa core complex that allows the Wiskott-Aldrich syndrome protein (WASH) function. FAM21C can be found in the membranes of early endosomes. FAM21C cooperates with CAPZ and hinders its actin-capping activity, links WASH to endosomes, and is required for WASH-dependent retromer-mediated sorting.

Immunogen

Recombinant protein corresponding to human WASH complex subunit FAM21C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 5 µg/mL of this antibody detected WASH complex subunit FAM21C in 10 µg of HEK293 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~145 kDa observed
Non-specific bands may be observed >200 kDa and at ~10 kDa