Application

Anti-FAM120A Antibody is an antibody against FAM120A for use in western blotting, IHC & immunofluorescence.

Research Sub CategoryRNA Metabolism & Binding Proteins

Research CategoryEpigenetics & Nuclear Function

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected FAM120A in rat brain tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected FAM120A in rat brain cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Constitutive coactivator of PPAR-gamma-like protein 1, also known as Oxidative stress-associated Src activator or Protein FAM120A, and encoded by the gene name FAM120A or OSSA, was recently detected within the Purα-containing mRNA-protein complex in the brain. As a novel RNA-binding protein, FAM120A is a critical component of the oxidative stress-induced survival signaling. It may participate in mRNA transport in the cytoplasm. FAM120A activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. FAM120A is highly expressed in scirrhous-type gastric cancer tissues compared with normal gastric mucosa. FAM120A protects cells from apoptosis through activation of SRKs in response to oxidative stress. Blocking of the survival signaling mediated by FAM120A, which sensitizes the cancer cells to stress-induced apoptosis, may be a novel therapeutic approach for gastric scirrhous carcinoma cells.

Immunogen

KLH-conjugated synthetic peptide corresponding to the N-terminus of human FAM120A.

Epitope: N-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in SK-N-SH cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected FAM120A in SK-N-SH cell lysate.

Specificity

This antibody will not cross-reacti with FAM120B/PGCC1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130 kDa observed. Uncharacterized bands may be observed in some lysate(s).