Application

Western Blotting Analysis: A representative lot detected ERLIN2/SPFH2 in HeLa cell lysates with or without deglycosylation by Endo H treatment (Pearce, M.M., et al. (2007). J Biol Chem. 282(28):20104-20115)
Western Blotting Analysis: A representative lot detected ERLIN2/SPFH2 in Rat-1 & HeLa cell lysates and various rat tissue homogenates, as well as IP3Rs activation-dependent increase of co-precipitated ERLIN2/SPFH2 in IP3Rs IP from rat gonadotroph pituitary alpha T3-1 cells and rat pancreatic tissue (Pearce, M.M., et al. (2007). J Biol Chem. 282(28):20104-20115).
Immunocytochemistry Analysis: A representative lot detected ERLIN2/SPFH2 ER localization in HeLa cells (Pearce, M.M., et al. (2007). J Biol Chem. 282(28):20104-20115).
Immunoprecipitation Analysis: A representative lot immunoprecipitated ERLIN2/SPFH2, as well as ERLIN2/SPFH2-associated IP3R1 and ERAD pathway proteins from rat pituitary gonadotroph alpha T3-1 cells (Pearce, M.M., et al. (2007). J Biol Chem. 282(28):20104-20115).

Research CategorySignaling

Research Sub CategorySignaling Neuroscience

This Anti-ERLIN-2 Antibody is validated for use in Western Blotting, Immunocytochemistry and Immunoprecipitation for the detection of SPFH2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Erlin-2 (UniProt B5DEH2; also known as Endoplasmic reticulum lipid raft-associated protein 2, SPFH domain-containing protein 2, SPFH domain family member 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2) is encoded by the Erlin2 (also known as RGD1309010, Spfh2) gene (Gene ID 290823) in rat species. Erlin2 (Spfh2) and the highly homologous Erlin1 (Spfh1) are ER proteins that hetero-oligomerize into a large complex of ∼2 MDa. The erlin1/2 complex associates with inositol 1,4,5-trisphosphate (IP(3)) receptors on ER membrane and is responsible for recruiting RNF170 upon IP(3) receptor activation for subsequent ubiquitination-dependent receptor downregulation.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of rat ERLIN-2/SPFH2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 155mM NaCl, 50mM Tris-HCl Ph 7.4 with 0.05% sodium azide and 10% glycerol.

Quality

Evaluated by Western Blotting in rat liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ERLIN-2/SPFH2 in 10 µg of rat liver tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~43 kDa observed