Anti-EPS8

Analysis Note

ControlNIH/3T3 cell lysate

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

This EPS8 antibody is validated for use in WB for the detection of the EPS8 protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

EPS8 (epidermal growth factor receptor kinase substrate 8), which was originally identified as a substrate for the kinase activity of EGFR, is a multifunctional protein that is known to play a role in spermatogenesis, the regulation of actin, and other cellular processes. EPS8 uses its SH3 domain to complex with Abi1 and SOS1, leading to Rac activation and subsequent actin cytoskeletal remodeling. In the same manner, it binds with RN-tre to inhibit the internalization of EGFR. EPS8 also works with Arp3 to modulate actin dynamics within the seminiferous epithelium at the apical ES and the BTB.

Immunogen

KLH-conjugated linear peptide corresponding to human EPS8.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected EPS8 in 10 µg of NIH/3T3 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~97 kDa observed. A second isoform is known to exist at ~68 kDa Uncharacterized bands may be observed at ~50 kDa and ~37 kDa in some cell lysates.