Analysis Note

ControlRat skeletal muscle tissue lysate

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected EPAC1 in human kidney, thalamus, and cerebellum tissues.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Anti-EPAC1 Antibody is a highly specific rabbit polyclonal antibody, that targets Epac & has been tested in western blotting & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Exchange protein directly activated by cAMP 1 (EPAC1) is also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I. EPAC1 is a guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases which are activated through cAMP binding. EPAC1 couples cAMP production to the activation of the Ras family GTPase, Rap. Activation of Rap drives several cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. EPAC1 is part of a signaling complex in which it activates the PI3K gamma complex, which is involved in angiogenesis. EPAC1 may also regulate ion channel formation, intracellular Ca++ signaling, ion transporter activity, and exocytosis. EPAC1 is widely expressed, especially in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal cord.

Immunogen

KLH-conjugated linear peptide corresponding to human EPAC1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in rat skeletal muscle tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected EPAC1 in 15 µg of rat skeletal muscle tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~103 kDa observed. Uncharacterized bands may appear in some lysate(s).