Application
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected ENTPD1 in 10 µg of human spleen and Jurkat cell lystaes.
Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected ENTPD1 in 1X10E6 Jurkat cells.
Research CategoryNeuroscience
Research Sub CategoryDevelopmental Signaling
This Anti-ENTPD1 Antibody is validated for use in Western Blotting, Flow Cytometry for the detection of ENTPD1.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Ectonucleoside triphosphate diphosphohydrolase 1 (EC:3.6.1.5; UniProt P49961; also known as CD39, Ecto-apyrase, Ecto-ATP diphosphohydrolase 1, Ecto-ATPDase 1, Ecto-ATPase 1, Lymphoid cell activation antigen, NTPDase 1) is encoded by the ENTPD1 (also known as CD39, SPG64) gene (Gene ID 953) in human. CD39 is a cell surface nucleotide-metabolizing enzyme that hydrolyzes extracellular purine NTP and NDP. CD39 is a putative marker for B-cell activation and its expression is also observed on NK-cells, dendritic cells, T-cells, and vascular endothelial cells. CD39 is a known inhibitor of ADP-induced platelet aggregation and may also play a role in angiogenesis. E. coli infection is reported to induce CD39 promoter activity in macrophages, resulting in an upregulated CD39 expression during sepsis in mice, while CD39 knockdown increases mortality among mice with polymicrobial sepsis.
Immunogen
Epitope: extracellular domain
KLH-conjugated linear peptide corresponding to the extracellular domain of human ENTPD1.
Other Notes
Concentration: Please refer to lot specific datasheet.
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality
Evaluated by Western Blotting in human placenta cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ENTPD1 in 10 µg of human placenta cell lysate.
Specificity
Expeccted to react with all six spliced isoforms, including the Vascular and the Placenta I&II isoforms.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
~65 kDa observed when using human placenta tissue lysates. Glycosylated target band(s) will appear larger than the calculated molecular weights of 58.0 kDa (Isoform 1/Vascular), 58.7 kDa ((Isoform 2/Placenta I), 34.2 kDa (Isoform 3/Placenta II), 42.7 kDa (Isoform 4), 46.2 kDa (Isoform 5), and 59.3 kDa (Isoform 6). Uncharacterized band(s) may appear in some lysates.
This product has met the following criteria to qualify for the following awards: