Analysis Note

ControlJurkat cell lysate

Application

Research CategoryApoptosis & Cancer

Detect ELMO1 using this Anti-ELMO1 Antibody validated for use in Western Blotting.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Engulfment and cell motility protein 1 (ELMO1) is a cytoplasmic protein involved in the clearance of apoptotic cells by phagocytosis. ELMO1 is activated downstream of the phosphatidylserine receptor BAI1. ELMO1 binds to DOCK180 and facilitates its transport to the plasma membrane where DOCK180 activates the GTPase RAC1, possibly via Arf6. RAC1, like other small GTPases, mediate cytoskeleton remodeling and cell motility which is necessary for the internalization of target cells.

Immunogen

Epitope: ELMO domain

KLH-conjugated linear peptide corresponding to human ELMO1 at the ELMO domain.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected ELMO1 on 10 µg of Jurkat cell lysate.

Specificity

This antibody recognizes ELMO1 at the ELMO domain.

No homology to Rat was found.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~78 kDa observed. This antibody exhibits strong affinity, high dilutions are recommended. The calculated molecular weight is 84 kDa, however ELMO1 has been shown as a ~78 kDa band in western blots (Gumienny, T.L., et al. (2001). Cell. 107:27–41.)