Analysis Note

ControlJurkat Cell Lysate3T3/A31 cell lysate

Application

Use Anti-EED Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EED also known as embryonic ectoderm development.

General description

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.

Immunogen

A synthetic peptide corresponding to a.a. 429-441 of human EED, conjugated to KLH.

Epitope: a.a. 429-441

Linkage

Replaces: 09-727

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Western Blot Analysis: 1 µg/mL of this lot detected EED on 10 µg of Jurkat cell lysate.

Specificity

This antibody recognizes EED, Mr ~ 62-70 kDa.

Target description

~53 kDa observed. Uniprot describes three isoforms at 51 kDa (isoform 1), 53 kDa (isoform 2), and 46 kDa (isoform 3).