07-1820 Display Image


Code: 07-1820 D2-231

Analysis Note

ControlBALB 3T3/A31 cell lysate


Research CategorySignaling

Research Sub CategoryGeneral Post-translation Modifica...

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Analysis Note

ControlBALB 3T3/A31 cell lysate


Research CategorySignaling

Research Sub CategoryGeneral Post-translation Modification

This Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1.

Western Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Early endosome antigen 1 (EEA1) is a 170 kDa coiled-coil protein belonging to a group of early endosomal Rab5 effector proteins. EEA1 has a putative role in the tethering/docking of endocytic vesicles prior to their fusion with early endosomes. This protein is characterized as containing a zinc finger-like domain and coiled-coil regions. The zinc finger-like domain, also known as the FYVE finger, is a motif rich in cystine, is a necessary component of EEA1's ability for endosomal targeting, and is specifically known for its dual interaction with Rab5 GTPase and phosphotidylinositol 3-phosphate (Ptdlns-3-P).


Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human EEA1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.


Evaluated by Western Blot in BALB 3T3/A31 cell lysate.

Western Blot Analysis: A 1:1000 dilution of this antibody detected EEA1 on 10 µg of BALB 3T3/A31 cell lysate.


This antibody recognizes EEA1.

Equine and Canine (99% sequence homology). Rat (98% sequence homology). Bovine (97% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 170 kDa

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcerabbit
Gene Informationhuman ... EEA1(8411)
NCBI accession no.NP_003557
packagingantibody small pack of 25 µL
purified byaffinity chromatography
Quality Level100
shipped inambient
species reactivityhuman, mouse
technique(s)immunocytochemistry: suitable, western blot: suitable
UniProt accession no.Q15075
Unit Size
List Price
List Price: €155.00
Source:List Price
Est. Dispatch/Availability