Application

Research CategoryStem Cell Research

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in PC12 cells. (Kishida et al., 2007. Dvl regulates endo- and exocytotic processes through binding to synaptotagmin. Genes to Cells, 12, 49–61).

Research Sub CategoryDevelopmental Signaling

This Anti-Dishevelled Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Dishevelled.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dishevelled (DVL1), the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation. The protein acts as a transducer molecule for developmental processes, including segmentation and neuroblast specification. Dishevelled plays a role both in canonical and non-canonical Wnt signaling. DVL1 mutation is likely responsible for transformation in neuroblastomas, and the Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1 gene, thus Dishevelled maybe important in these diseases as well.

Immunogen

GST-tagged recombinant protein corresponding to Human Dishevelled.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in HEK293T cell lysate.

Western Blot Analysis: A 1:1000 of this antibody detected Dishevelled in 10 µg of HEK293T cell lysate.

Specificity

Recognizes Human, Mouse and Rat Dishevelled 1, 2 and 3.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~ 81 kDa observed