Analysis Note

ControlHepG2 cell lysate

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected DLK1 in mouse embryonic tissue.

Research CategoryNeuroscience

This Anti-DLK1 Antibody is validated for use in WB, IH for the detection of DLK1.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Delta-like 1 homolog (DLK1), also known as fetal antigen 1 (FA1) and preadipocyte factor 1 (pref-1), is a transmembrane protein containing 6 epidermal growth factor repeats whose expression is known to modulate differentiation signals. Studies have shown DLK1 is involved in differentiation of several cell types, including adipocytes. Studies have shown deficiencies in DLK1 displaying growth retardation, obesity, skeletal malformation, and increased serum lipid. DLK1 is a paternally expressed imprinted gene that plays a role in normal development and in the maintence of homeostatsis of adipose tissue mass.

Immunogen

KLH-conjugated linear peptide corresponding to human DLK1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DLK1 on 10 µg of HepG2 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~47 kDa observed. The calculated molecular weight of this protein is 41 kDa, but can be observed at ~41-55 kDa due to high glycosylation.