Analysis Note

ControlHEK293 cell lyaste

Application

Research Sub CategoryPI3K, Akt, & mTOR Signaling

Research CategoryMetabolism

Anti-DEPTOR Antibody detects level of DEPTOR & has been published & validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

DEPTOR (DEPDC6, DEP domain-containing protein 6) was recently identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. DEPTOR negatively regulates mTORC1 and mTORC2. It has been shown that loss of DEPTOR activates various targets that include p70S6K1, Akt, and SGK1, that leads to cell growth and survival. Little was known about DEPDC6 prior to the discovery of its interactions with mTOR, at which time it was given the new name of DEPTOR (Peterson, T.R., et al. (2009). Cell. 137(5):873-876).

Immunogen

KLH-conjugated linear peptide corresponding to the DEP domain-containing mTOR-interacting protein of human DEPTOR.

Epitope: DEP domain-containing mTOR-interacting protein DEPTOR.

Linkage

Replaces: 09-463

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DEPTOR in HEK293 cell lysate.

Specificity

Other homologies: Primate, Equine, Porcine, Canine, Rat, and Mouse based on 99%, 99%, 98%, 97%, and 96% homology, respectively.

This antibody recognizes the DEP domain that contains mTOR-interacting protein DEPTOR.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~46 kDa observed.