Analysis Note

ControlHuman kidney tissue lysate

Application

Anti-Cystatin-C Antibody detects level of Cystatin-C & has been published & validated for use in WB, IH(P).

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - AdditionalNeurodegenerative Diseases

Immunohistochemistry (paraffin) Analysis: A 1:1000 dilution of a previous lot detected Cystatin-C in purkinje cells of normal rat cerebellum, neurons of normal rat frontal lobe, and neurons in normal rat midbrain.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cystatin-C is a cysteine protease inhibitor in mammals, which is essential in protein degradation and keeping an equilibrium between inhibitors and proteases. It is thought to be a better marker for renal dysfunction and potential kidney injury than creatinine. Recently, Cystatin-C has been implicated in primary hypertension and may be a novel marker in patients. Cystatin-C has also been implicated in oxidative stress-induced apoptosis of CNS neurons and has been shown to inhibit amyloid-beta deposition in Alzheimer′s disease models.

Immunogen

KLH-conjugated linear peptide corresponding to human Cystatin-C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Cystatin-C in 10 µg of human kidney tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~14 kDa observed.
An uncharacterized band may appear at ~62 kDa in some lysates.