Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Capicua in human cerebral cortex tissue.

This Anti-Capicua Antibody is validated for use in Western Blotting for the detection of Capicua.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein capicua homolog (CIC) , also known as Capicua Transcriptional Repressor, Capicua Homolog, and encoded by the gene CIC, is a transcriptional repressor which plays a role in development of the central nervous system (CNS). CIC is a new subfamily of the HMG-box superfamily and the gene′s expression is predominantly restricted to immature granule cells in the cerebellum, hippocampus and olfactory bulb in the CNS, and thus the gene is implicated in CNS development, in particular in granule cell development. CIC appears to be central to cerebellar development and ErbB signaling as well. Recent research has demonstrated that CIC is highly expressed in certain cancers including medulloblastomas, but also the loss of expression may serve as a biomarker for other cancer types such as oligodendrogliomas.

Immunogen

Epitope: Near N-terminal

KLH-conjugated linear peptide corresponding to human Capicua near the N-terminal.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human cerebellum tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Capicua in 10 µg of human cerebellum tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~160 kDa observed. Long isoform can be detected at ~250kDa with longer exposure, in some lysates (Kim, E., et al. (2013). Genea Dev. 27:590-595).