Analysis Note

ControlMouse heart tissue lysate

Application

Research CategoryApoptosis & Cancer

Detect CIDE-A using this rabbit polyclonal antibody, Anti-CIDE-A Antibody validated for use in western blotting, IHC & Immunofluorescence.

Research Sub CategoryApoptosis - Additional

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected CIDE-A in mouse heart tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected CIDE-A in mouse heart tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cell death activator CIDE-A (CIDE-A) binds to lipid droplets and regulates their enlargement which restricts lipolysis and favoring storage. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. CIDE-A is highly expressed in brown adipose tissue and, at lower levels, in white adipose tissue.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of mouse CIDE-A.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CIDE-A in 15 µg of mouse heart tissue lysate.

Specificity

This antibody recognizes the C-terminus of CIDE-A.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~25 kDa observed