Application

Anti-CHD-8 | ABN1029 is an antibody against CHD-8 for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Western Blotting Analysis: A representative lot detected CHD-8 in A431 cell lysate (Sakamoto, I., et al. (2000). JBC. 275:32871-32878).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CHD-8 in β-catenin cell lysate (Sakamoto, I., et al. (2000). JBC. 275:32871-32878).
Immunocytochemistry Analysis: A representative lot detected CHD-8 in Lcells and SW480 cells (Sakamoto, I., et al. (2000). JBC. 275:32871-32878).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CHD-8 is a DNA helicase that acts as a chromatin remodeling factor as well as a transcriptional regulator. CHD-8 acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to various genes such as p53. CHD-8 regulates beta catenin activity by suppressing the promoter activity of several beta catenin targeted genes. CHD-8 also suppresses STAT3 transcriptional activation in a similar fashion. CHD-8 is a component of multiple chromatin complexes and localizes to the nucleus and active chromatin. Interestingly mutations in CHD-8 are associated with a form of autism, Autism 18 as well as some forms of developmental delay and cognitive impairment, demonstrating CHD-8's potential importance in developmental brain function, and behavior.

Immunogen

Recombinant protein corresponding to rat CHD-8.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CHD-8 in 10 µg of HepG2 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~290 kDa observed. Uncharacterized band(s) may appear in some lysates.