Analysis Note

ControlHeLa cell lysate

Application

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Anti-CHD-3 Antibody is a Rabbit Polyclonal Antibody for detection of CHD-3 also known as Chromodomain-helicase-DNA-binding protein 3, hZFH & has been validated in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Chromodomain-helicase-DNA-binding protein 3, more commonly known as CHD-3, is a member of the CHD family of proteins. These proteins are characterized by the presence of chromo (chromatin organization modifier) domain, SNF2-related helicase/ATPase domain, and and a carboxy-terminal DNA binding domain. As a component of the Mi-2/NuRD histone deacetylase complex, CHD-3 participates in chromatin remodeling by deacetylating histones.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human CHD-3.

Epitope: C-terminus

Linkage

Replaces: MABE482

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected CHD-3 on 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes CHD-3 at the C-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 250 kDa observed