Anti-Bystin

Analysis Note

ControlHeLa cell lysate

Application

Research CategoryNeuroscience

Anti-Bystin Antibody is an antibody against Bystin for use in WB, IH(P).

Research Sub CategoryAdhesion (CAMs)

Immunohistochemistry Analysis: A 1:300 dilution of this antibody detected Bystin in the syncytiotrophoblastic layer of the chorionic villi in human placenta.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bystin and tastin serve as cytoplasmic proteins that are required for trophinin to mediate cell-to-cell adhesion between trophoblastic and endometrial epithelial cells in human placenta. Although the trophinin gene is unique to mammals, the bystin gene (BYSL) is conserved across eukaryotes. It is shown that bystin influences the transition from silent trophectoderm to an active trophoblast through trophinin-mediated cell adhesion. It is also essential for embryonic stem cell survival and trophectoderm development in mouse. Bystin is demonstrated to play a key role in ribosomal biogenesis by processing 18S RNA in the 40S subunit in human cancer cells and mouse embryos. It is also reported that c-MYC targets BYSL, which corresponds with bystin-mediated, rapid protein synthesis, and subsequent cellular growth.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human Bystin.

Epitope: C-terminus.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Bystin in 10 µg HeLa cell lysate.

Specificity

This antibody recognizes the C-terminus of Bystin.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~55 kDa observed. UniProtKB/Swiss-Prot Q13895 (BYST_HUMAN) states a molecular weight of ~49 kDa