Analysis Note

ControlHuman bone marrow tissue lysate

Application

Research CategoryApoptosis & Cancer

Anti-BST2 is an antibody targeting the BST2 protein, validated for use in WB & IHC.

Research Sub CategoryApoptosis - Additional

Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected BST2 in human cardiac myocyte and human hepatocyte tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

BST-2 also known as CD317 and Tetherin, bone marrow stromal antigen 2 (BST-2) is an interferon-inducible transmembrane protein that potently inhibits the release of nascent retrovirus particles. BST-2 is downregulated by the viral Vpu protein. BST-2 acts as a direct physical tether, which holds and links virions at the cell membrane. These tethered virons can remain on the cell surface or be internalized by endocytosis for degradation. BST-2 inhibits MMP2 and MMP15 activity, resulting in the attenuation of cellular proliferation and migration. BST-2 interaction with LILRA/ILT7 receptors in plasmacytoid dendritic cells negatively regulates IFN production in response to viral infection.

Immunogen

Epitope: extracellular domain

KLH-conjugated linear peptide corresponding to the extracellular domain of human BST2.

Linkage

Replaces: MABF201

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human bone marrow tissue lysate.

Western Blot Analysis: 1.0 µg/mL of this antibody detected BST2 in 10 µg of human bone marrow tissue lysate.

Specificity

This antibody recognizes the extracellular domain of BST2

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~33 kDa observed. The calculated molecular weight of this protein is 20 kDa, but can be observed at ~25-35 kDa due to high glycosylation.