Analysis Note

ControlNon-transfected, RISC free HEK293 cell lysate and Atg13 siRNA knockdown HEK293 cell lysate.

Application

Anti-Atg13 Antibody is a highly specific rabbit polyclonal antibody, that targets ATG13 & has been tested in western blotting.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Western Blotting: A representative lot detected Atg13 in ATG13 transfected HEK293A cell lysates (Chan, E. Y., et al. (2009). Mol Cell Biol. 29(1):157-171.).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Autophagy-related protein 13 (ATG13) is an autophagy factor necessary for autophagosome formation. ATG13 is targeted by the TOR kinase signaling pathway which regulates autophagy by controlling ATG13 phosphorylation status and regulating the ATG13-ULK1-RB1CC1 complex.

Immunogen

Epitope: C-terminus

Linear peptide corresponding to the C-terminus of human Atg13.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in non-transfected, RISC free HEK293 cell lysate and Atg13 siRNA knockdown HEK293 cell lysate.

Western Blotting: A 1:1,000 dilution from a representative lot detected Atg13 in non-transfected, RISC free HEK293 cell lysate and demonstrated a loss of signal in Atg13 siRNA knockdown HEK293 cell lysate.

Specificity

This antibody recognizes the C-terminus of Atg13.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~57 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.