Application

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected detected Arpin and C15orf38-AP3S2 in MEF-1 cells.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

This Anti-Arpin Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of Arpin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Arpin (UniProt Q7Z6K5; also known as Arp2/3 inhibition protein, UPF0552 protein C15orf38) is encoded by the ARPIN (also known as C15orf38) gene (Gene ID 348110) in human. Cell migration requires the generation of branched actin networks that power the protrusion of the plasma membrane in lamellipodia. The actin-related proteins 2 and 3 (Arp2/3) complex nucleates actin filaments by binding to the side of an existing filament and initiating branch formation. Arpin functions as a negative regulator of Arp2/3 activity, allowing cells to control directional persistence of migration by fine-tuning actin nucleation activity at the leading edge of the lamellipodium. Arpin depletion in both mammalian cells and Dictyostelium discoideum amoeba is reported to result in straighter trajectories. Arpin microinjection is demonstrated to induce fish keratocytes to turn.

Immunogen

Epitope: near Arpin C-terminus

KLH-conjugated linear peptide corresponding to human Arpin near the C-terminus.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in MEF-1 murine embryonic fibroblasts cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected detected Arpin and C15orf38-AP3S2 in 10 µg of human U2OS and murine MEF-1 embryonic fibroblasts cell lysate.

Specificity

Reacts with both Arpin and the C15orf38-AP3S2 fusion (isoform 2) produced from a naturally occurring readthrough transcript.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~28/42 kDa observed (24.9 kDa/Arpin and 43.9 kDa/C15orf38-AP3S2 calculated)