Application

Research CategorySignaling

Research Sub CategorySignaling Neuroscience

Anti-Angiomotin Antibody | ABS1023 is an antibody against Angiomotin Antibody for use in Western Blotting, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP).

Western Blotting Analysis: A representative lot detected phospho Angiomotin (Ser175) in HEK293 transfected with AMOT cell lysate (Dr. Joseph Kissil, Scripps Research Institute, Florida).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Angiomotin in HEK293 cells expressing equal amount of HA-Merlin and Myc-Rich1, untreated (−) or Doxycyline induced (+) RT4-67 cells (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).
Western Blotting Analysis: A representative lot detected Angiomotin in Flag-IP and total cell lysate from HEK293 cells transfected with Flag-tagged wild-type (WT) or patient-derived mutant Merlin alleles, HEK293, ERK activation in Nf2−/− SC4 cells or Merlin-reconstituted SC4 cells (Nf2−/−+retroNf2) (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).
Western Blotting Analysis: A representative lot detected Angiomotin in HEK293, MEFs treated with Adeno (−) or
Adeno-Cre (+) virus (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
Immunofluorescence Analysis: A representative lot detected Angiomotin in epithelial cells (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).
Immunofluorescence Analysis: A representative lot detected Angiomotin in liver from Amot KO mice (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
Immunocytochemistry Analysis: A representative lot detected Angiomotin in SC4 cells (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Angiomotin in HEK293 cells (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Angiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemotaxis, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein complex associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is expressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes.

Immunogen

Linear peptide corresponding to human Angiomotin.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HEK293 transfected with LATS cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Angiomotin in 10 µg of HEK293 transfected with LATS cell lysate.

Specificity

This antibody detects p80 and p130 forms of AMOT

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

150/80 kDa observed