Analysis Note
ControlHEK293 cell lysate
Application
Research CategorySignaling
Research Sub CategoryDevelopmental Signaling
Anti-ASAH2 Antibody is a highly specific rabbit polyclonal antibody, that targets ASAH2 & has been tested in western blotting.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
N-acylsphingosine amidohydrolase 2 (ASAH2), also known as neutral ceramidase (N-CDase, NCDase), is a key regulator of sphingolipid signaling metabolites. ASAH2 is known to hydrolyze the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Additionally, ASAH2 is suggested to act as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. ASAH2 is also suggested to be involved in the digestion of dietary sphingolipids in intestine tract. In this role, ASAH2 is implied to act as a key enzyme for the catabolism of dietary sphingolipids and consequently, regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. ASAH2 is expressed primarily in the intestine. It is also expressed with higher levels in kidney, skeletal muscle and heart.
Immunogen
KLH-conjugated linear peptide corresponding to human ASAH2.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected ASAH2 in 15 µg of HEK293 cell lysate.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
~70 kDa observed. Uniprot describes 2 isoforms at ~82 kDa and ~86 kDa Uncharacterized bands may appear in some lysate(s).
This product has met the following criteria to qualify for the following awards: