Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected ARC in human heart and skeletal muscle tissues.

Anti-ARC Antibody detects level of ARC & has been published & validated for use in ARC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nucleolar protein 3 (NOL3), also known as Apoptosis repressor with CARD or Muscle-enriched cytoplasmic protein (Myp) or Nucleolar protein of 30 kDa (Nop30) and encoded by the gene name NOL3 or ARC contains one CARD domain. Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and –8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to amino acids 191-208 at the C-terminus of human ARC.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in HeLa, KB, and A549 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARC in HeLa, KB, and A549 cell lysates.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~24 kDa observed. Uncharacterized bands may be observed in some lysate(s).