Analysis Note

ControlHuman spleen tissue lysate

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Apobec1 in rat small intestine and human colon tissue.

Detect Apobec1 using this rabbit polyclonal antibody, Anti-Apobec1 Antibody validated for use in western blotting & IHC (Paraffin).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Apolipoprotein B mRNA-editing enzyme 1 (Apobec1) is also known as C->U-editing enzyme APOBEC-1, and HEPR. Apobec1 is a member of the cytidine and deoxycytidylate deaminase family that is exclusively expressed in the small intestine. Apobec1 is a catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for postranscriptional mRNA editing. Apobec1 is also thought to be involved in the epigenetic regulation of gene expression by participating in DNA demethylation.

Immunogen

KLH-conjugated linear peptide corresponding to human Apobec1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Apobec1 in 10 µg of human spleen tissue lysate.

Specificity

Other homologies: Mouse (79% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~28 kDa observed