Analysis Note

ControlMouse brain tissue lysate.

Application

Research CategorySignaling

This Anti-ADAP1 Antibody is validated for use in Western Blotting, IHC(P) for the detection of ADAP1.

Immunohistochemistry Analysis: A representative lot detected ADAP1 in normal mouse cerebellum and hippocampus tissues.

Western Blot Analysis: A representative lot detected ADAP1 in human brain tissue lysate.

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Arf-GAP with dual PH domain-containing protein 1 (ADAP1), also known as p42(IP4), PIP3BP, or centaurin-alpha1, is a highly abundant brain protein that has been detected in the hippocampus, cerebellum, cortex, and hypothalamus. It is an actin-binding protein that has been localized to the nucleus, cytoplasm, plasma membrane, and mitochondria. ADAP1 contains a zinc finger motif and two pleckstrin homology domains. It functions as a receptor for inositol(1,3,4,5)tetrakisphosphate and phosphatidylinositol(3,4,5)trisphosphate second messengers, and also binds to and becomes phosphorylated by all isoforms of PKC. It has been linked to calcium transport in the mitochondria and RNA metabolism in the nucleus. In addition, it has been shown that ADAP1 modulates the development and differentiation of dendrites in hippocampal cells, as it is involved in the formation of dendritic filopodia and spine-like protrusions. It has also been reported that ADAP1 is upregulated in Alzheimer’s disease.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human ADAP1.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1 mg/mL of this antibody detected ADAP1 in 10 µg of mouse brain tissue lysate.

Specificity

This antibody recognizes the C-terminus of ADAP1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~44 kDa observed