Analysis Note

ControlHEK293 cell lysate

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Immunocytochemistry Analysis: 5 µg/mL from a representative lot detected ABCA7 in HEK293 cells.

Anti-ABCA7 Antibody is a highly specific rabbit polyclonal antibody, that targets ABCA7 & has been tested in western blotting & ICC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ATP-binding cassette sub-family A member 7 (ABCA7), also known as ABCA-SSN and Macrophage ABC transporter, is a full-size ABC transporter consisting of two sets of the multiple membrane-spanning domains plus the Walker motifs for the ATP interaction. It uses ATP hydrolysis to catalyze the transport of various molecules across cell membranes. ABCA7 is involved in phagocytosis by macrophages of apoptotic cells, binds to APOA1, may function in phospholipid and cholesterol efflux from cells, and may regulate cellular ceramide homeostasis during keratinocyte differentiation The high ABCA7 expression levels in peripheral leukocytes, thymus, spleen and bone marrow indicates a role in the immune system lipid homeostasis. ABCA1 is also highly expressed in fetal tissues, especially the liver.

Immunogen

KLH-conjugated linear peptide corresponding to human ABCA7.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1µg/mL of this antibody detected ABCA7 in 15 µg of HEK293 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~234 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~219 kDa and ~234 kDa