Anti-3-PGDH

Application

Research CategorySignaling

This Anti-3-PGDH Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of 3-PGDH.

Research Sub CategorySignaling Neuroscience

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected 3-PGDH in human cerebral cortex, human kidney, and human cerebellum tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

3-PGDH (PHGDH) protein in an important enzyme in L-serine biosynthesis. 3-PGDH performs the first rate limiting step in the phosphorylated pathway of serine biosynthesis. 3-PGDH is also a biomarker in certain cancers that over express 3-PGDH. Moreover, 3-PGDH is the essential enzyme needed for cells to traverse the S phase and divide, and it plays a key role in T cell activation and proliferation for instance. Interestingly, even though 3-PGDH knockdown and reduced serine synthesis slows growth and proliferation of many cancer cells in vitro 3-PGDH knockdown does not affect tumor maintenance and growth in established breast cancer xenograft models, suggesting that 3-PGDH-dependent cancer cell growth may be context-dependent.

Immunogen

KLH-conjugated linear peptide corresponding to human 3-PGDH near the N-terminus.

Epitope: Near N-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected 3-PGDH in 10 µg of A431 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~54 kDa observed