APN-COCI

Application

APN-COCl is a bifunctional reagent appropriate for the introduction of APN function.

Other Notes

The coupling between an amine and APN-COCl should be performed in dry organic solvents (e.g. THF) in the presence of a base (e.g. TEA). The resulting conjugate can be readily coupled with thiol-containing biomolecules using the following protocol: Dissolve the protein in the appropriate buffer with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration. Apply the appropriate amount of the stock solution of the APN-labeled molecule (1-5 molar eq. per free cysteine residue). Incubate at room temperature for 2 hours.If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

Packaging

50 mg in glass bottle