Analysis Note

ControlEGF treated and untreated A431 cell lysates, PDGF treated and untreated NIH/3T3 cell lysates, pervanadate treated and untreated PC12 cell lysates.

Application

Research Sub CategoryTranscription Factors

Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2 detects level of Phospho-Stat1 (Tyr701) & has been published & validated for use in WB.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

STATs (Signal-Transducing Activators of Transcription) are the principle substrates of JAK kinases, and mediators of cytokine signaling. STAT proteins have SH2 domains, and once phosphorylated by JAK kinases, STATs dimerize in a head-to-tail fashion, through their SH2 domains. STAT1 is a critical mediator of IFN signaling, STAT4 is specific to IL-12, STAT6 is activated primarily by IL-4 and IL-13, while STAT5 A and B appear to be most important to the function of growth hormone and prolactin. STAT3 is involved in multiple signaling pathways, and mice deficient in it are not viable. In addition to tyrosine phosphorylation, many STAT proteins are targets of Ser/Thr kinases, and those phosphorylation events potentiate STAT-induced transcription. The phosphorylation state of STATs can be monitored by phosphorylation state-specific antibodies.

Immunogen

KLH-conjugated linear peptide corresponding to the region around phosphorylated tyrosine 701.

Epitope: Phosphorylated Tyr701

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS and 0.05% sodium azide.

Format: Purified

Quality

Routinely evaluated by western blot on EGF treated and untreated A431 cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this lot was used to detect STAT1 phosphorylated at Tyr701 in EGF treated and untreated A431 cell lysates.

Specificity

This antibody recognizes phosphorylated STAT1 at tyrosine 701.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

88-92 kDa