Analysis Note

ControlMouse embryo e11 tissue lysate

Application

Research Sub CategoryTranscription FactorsDevelopmental Signaling

Anti-ZEB1 detects levels of ZEB1 proteins & has been published & validated for use in WB.

Research CategoryEpigenetics & Nuclear FunctionNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Zinc finger E-box-binding homeobox 1 (ZEB1), also known as Transcription factor 8 (TCF8) and Negative regulator of IL2 or NIL-2-A zinc finger protein is a member of the Zinc finger-Homeobox (ZFH)-family of repressors and acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. ZEB1 has also been implicated in regulating the expression of ATP1A1, E-cadherin, BCL6, and RCOR1 genes. ZEB1 regulates neurogenesis and neuronal differentiation, but is also implicated in tumor formation. ZEB1 is expressed in heart and skeletal muscle. Mutations in ZEB1 have been associated with corneal dystrophy.

Immunogen

KLH-conjugated linear peptide corresponding to human ZEB1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse embryo e11 tissue lysate.

Western Blot Analysis: 1.0 µg/mL of this antibody detected ZEB1 in 10 µg of mouse embryo e11 tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~200 kDa observed. The calculated molecular weight is 124 kDa, however ZEB1 has been shown at ~180-210 kDa in western blots (Dave, N., et al. (2011). J. Biol. Chem. 286(14):12024-12032).