Application

Western Blotting Analysis: A representative lot detected TTC39B in cultured primary mouse hepatocytes transfected with T39 (Hsieh, J., et. al. (2016). Nature. 535(7611):303-7).

Anti-TTC39B, Cat. No. ABS2119, is a rabbit polyclonal antibody that detects Tetratricopeptide repeat protein 39B and has been tested for use in Western Blotting.

General description

Tetratricopeptide repeat protein 39B (UniProt: also known as TPR repeat protein 39B, TTC39B) is encoded by the TTC39B (also known as C9orf52) gene (Gene ID: 158219) in human. TTC39B protein contains two TPR repeats (aa 393-426 and 626-659). TCC39B protein is the product of a high density lipoprotein (HDL) gene that promotes the ubiquitination and degradation of liver X receptor (LXR). TTC39B null or deficient mice challenged with high fat/cholesterol/bile salt diet exhibit increased LXR protein and target gene expression. TTC39B deficiency is reported to stabilize LXR by reducing its polyubiquitination and proteasomal degradation. Hence, it reduces the incidence of atherosclerosis and steatohepatitis. TTC39B deficient mice display higher levels of HDL and apoplipoprotein A-1 and lower levels of hepatic triglyceride synthesis. However, it does not affect glucose tolerance or hepatic gluconeogenesis. (Ref.: Hsieh, J et al. (2016). Nature 535(7611): 303-307).

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Tetratricopeptide repeat protein (TTC39B).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in HEK293T cells transfected with TTC39B.

Western Blotting Analysis: 1 µg/mL of this antibody detected TTC39B in 10 µg of TTC39B transfected HEK293T cell lysate.

Specificity

This rabbit polyclonal antibody detects TTC39B in human and murine cells. It targets an epitope with in 20 amino acids from the C-terminal half.

Target description

~90 kDa observed; 76.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).