Analysis Note

ControlRat brain tissue lysate

Application

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected TMEM18 in mouse brain tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected TMEM18 in mouse brain tissue.

Research CategoryNeuroscience

Research Sub CategorySensory & PNS

Anti-TMEM18 Antibody is a highly specific rabbit polyclonal antibody, that targets TMEM18 & has been tested in western blotting, IHC & Immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Transmembrane protein 18 (TMEM18) is a transcription repressor and cell migration modulator which enhances the glioma-specific migration ability of neural stem cells (NSC) and neural precursor cells (NPC). Recent studies have shown that overexpression of TMEM18 provides NSCs and neural precursors an increased migration capacity towards glioblastoma cells, while functional inactivation of the gene resulted in an almost complete loss of migration activity. This evidence implies that TMEM18 could be a potential target in NSC-based glioma therapy. Other studies suggest that TMEM18 loci is associated with higher than normal body mass index (BMI) and obesity, and may play a CNS-related role in predisposition to obesity.

Immunogen

KLH-conjugated linear peptide corresponding to human TMEM18.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TMEM18 in 15 µg of rat brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~27 kDa observed. Uniprot describes 2 isoforms at ~15 kDa and ~16 kDa