Analysis Note

ControlRat brain tissue lysate

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Detect Synaptopodin using this rabbit polyclonal antibody, Anti-Synaptopodin Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected SYNPO in human brain tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Synaptopodin (SYNPO) is an actin-associated protein that may play an important role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. It is expressed in the cerebral cortex. Mutations in SYNPO may be associated with nephrotic syndrome.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human SYNPO.

Epitope: Near C-terminus.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified.

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected SYNPO in 15 µg of rat brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~95 kDa observed. Uniprot describes three isoforms at ~74 kDa, ~96 kDa, and ~97 kDa Uncharacterized bands may appear in some lysates.