Analysis Note

ControlMouse embryo pancreas tissue

Application

Use Anti-Sox4 Antibody (Rabbit Polyclonal Antibody) validated in IHC to detect Sox4 also known as SRY (sex determining region Y)-box 4, ecotropic viral integration site 16.

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Western Blotting: Using 1:1000 dilution of a representative lot of this antibody and lysate of human pancreas, a faint band was observed at ~48 kDa with a stronger uncharacterized band observed at ~38 kDa.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Sox4 is a protein transcription factor belonging to a family of transcription factors consisting of well over 14 members and 7 different subgroups. Members of the Sox family are characterized by DNA binding domains showing strong similarity to HMG box domains.
Proteins belonging to the Sox family are involved in various phases of embryonic development including; neural development, hemopoiesis, lens development, chondrogenesis, and sex determination as well as cell fate. Sox4 is a part of the C subgroup of Sox proteins, and is involved in B cell and endocardial ridge development. During embryonic development, Sox4 expression has been observed in several tissues including; lung, gonads, embryonic heart, mesonephros, CNS, thymus, tooth buds, and T and pre-B cells. Sox4 has also been observed in mature tissue, especially in the pancreas, heart, lung, CHS, thymus, and gonads. Recent studies have linked overexpression of Sox4 to the development of various types of tumors and cancers, and indicating a potential for Sox4 as a therapeutic marker.

Immunogen

GST-tagged recombinant protein corresponding to mouse Sox4.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Quality

Evaluated by Immunohistochemistry in mouse embryo pancreas tissue.

Immunohistochemistry Analysis: 1:300 dilution of this antibody detected Sox4 in mouse embryo pancreas tissue.

Specificity

This antibody recognizes Sox4.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

Calculated MW ~45 kDa