Application

Research CategoryEpigenetics & Nuclear Function

Anti-SIRT4 Antibody detects level of SIRT4 & has been published & validated for use in SIRT4.

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NAD-dependent protein deacetylase sirtuin-4 (SIRT4) is also called NAD-dependent ADP-ribosyltransferase sirtuin-4 and SIR2-like protein 4 (SIR2L4). SIRT4 catalyzes the transfer of ADP-ribosyl groups onto target proteins, and has been shown to inhibit GLUD1 enzyme activity, down-regulate insulin secretion and is involved in chromatin silencing. SIRT4 is believed to be a transcriptional regulator in embyonic stem cell and hematopoietic stem cells. SIRT4 is widely expressed and has been detected in vascular smooth muscle and striated muscle, and pancreatic (islets of Langerhans) insulin-producing beta-cells.

Immunogen

KLH-conjugated synthetic peptide corresponding to the N-terminus of human SIRT4.

Epitope: N-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SIRT4 in human liver tissue lysate.

Specificity

This antibody is predicted to not cross-react with other Sirtuin family members.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~38 kDa observed. Uncharacterized bands may be observed in some lysate(s).