Analysis Note

ControlHeLa nuclear extract

Application

Research Sub CategoryTranscription FactorsSignaling Neuroscience

Research CategoryEpigenetics & Nuclear Function

This Anti-REST Antibody is validated for use in WB for the detection of REST.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

REST (RE1-silencing transcription factor) also known as NRSF (neuron-restrictive silencer factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). The binding of REST to the NRSE represses neuronal gene transcription in non-neuronal cells. Although REST is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain. REST contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus. REST associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for REST outside the nervous system. Down-regulation of REST, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons. REST is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.

Immunogen

GST fusion protein corresponding to residues 801-1097 of human REST.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified rabbit serum in PBS with 0.07% sodium azide, and 30% glycerol.

Antigen Affinity Purified

Quality

Evaluated by western blot with HeLa nuclear extract.

Western Blot Analysis:
0.5-1 µg/mL of this antibody detected REST in nuclear extracts from HeLa, Raji, and Jurkat cells.

Specificity

Recognizes REST

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

120 kDa