Application

Research Sub CategoryNeuronal & Glial Markers

Research CategoryNeuroscience

Anti-Radial Glial Cell Marker-2 Antibody, clone RC2 detects level of Radial Glial Cell Marker-2 & has been published & validated for use in IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The monoclonal antibody Radial Glial Cell Marker-2, clone RC2 (RC2) was generated in mouse by conventional hybridoma methodology. The antigen recognized by RC2 is robust, allowing aldehyde fixation appropriate to high resolution light and electron microscopic analyses. From the neural tube stage of fetal development the antibody delineates throughout the central nervous system a subpopulation of neuroepithelial cells which have a radial bipolar morphology. RC2 also recognizes monopolar cell forms in the spinal cord and the cerebellum as early as E15, and in the dentate gyrus of the hippocampal formation from the day of birth. Monopolar forms in the cerebellum are inferred to be progenitors of Bergmann glia. The robustness of the antigen recognized by RC2 makes this probe a valuable tool to study the morphological transformations of the bipolar radial glia during their mitotic turnover. It also provides a sensitive stain for the study of the organization and the histogenetic role of the overall radial fiber system (3).

Immunogen

Embryonic rat brain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ammonium Sulfate Precipitation

Ammonium sulfate purified mouse monoclonal in PBS with 0.05% NaN₃.

Format: Purified

Specificity

Other species have not been tested.

Detects radial glial cell marker-2

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.