Analysis Note

ControlHeLa S3 cell lysate

Application

Research CategoryApoptosis & Cancer

Detect PRUNE using this Anti-PRUNE Antibody validated for use in Western Blotting.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein prune homolog, otherwise known as PRUNE or Drosophila-related expressed sequence 17, is a phosphodiesterase enzyme belonging to the PPase class C family. PRUNE targets cAMP and cGMP second messengers and plays a role in cell proliferation and motility. It is overexpressed in various sarcomas, including leiomyosarcomas, malignant fibrous histiocytomas, and malignant liposarcomas.

Immunogen

KLH-conjugated linear peptide corresponding to human PRUNE.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa S3 cell lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected PRUNE in 10 µg of HeLa S3 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~57 kDa observed