Anti-Prickle1

Code: ABN1699 D2-231

Application

Research CategoryNeuroscience

Western Blotting Analysis: A representative lot detected Prickle1 in Western Blotting applications (Liu, C., et. al. (2014). B...


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€328.00 EACH
€403.44 inc. VAT

Application

Research CategoryNeuroscience

Western Blotting Analysis: A representative lot detected Prickle1 in Western Blotting applications (Liu, C., et. al. (2014). Biol Open. 3(9):861-70).

Anti-Prickle1 , Cat. No. ABN1699, is a highly specific rabbit polyclonal antibody that targets Prickle-like protein 1 and has been tested in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Prickle-like protein 1(UniProt: Q3U5C7) is encoded by the Prickle1 (also known as Pk1) gene (Gene ID: 106042) in murine species. Prickle Prickle-like Protein 1 is involved in the planar cell polarity (PCP) pathway that controls convergent extension during gastrulation and neural tube closure. PCP pathway comprises of multiple integral membrane proteins ((Frizzled, Flamingo/Stan/Celsr and Vangl/ Strabismus) and membrane-associated proteins (Dishevelled, Inversin, and Prickle). Prickle-like protein 1 is synthesized with a propeptide (aa 830-832) that is removed in mature protein. Prickle1 is expressed in distinct cell populations of the central nervous system and is a contributor to neuronal morphogenesis. Both Pk1 and Pk2 genes share three LIM (Lin 11, Isl-1 and Mec-3) and one PET domain (Prickle, Espinas and Testin) at the N-terminus of the protein and a CAAX prenylation motif at the C-terminus. Pk1 is shown to be expressed in the cortical subplate and is considered as an important site for early neuronal maturation. In the retinal tissue Pk1 expression is specific to neurons and is highly enriched in Starburst amarcine cells. Loss of Pk1 gene has been linked to early embryonic lethality.

Immunogen

His-tagged Recombinant Protein corresponding to the C-terminus of Mouse Prickle-like protein 1.

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in mouse retinal tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Prickle1 in mouse retinal tissue lysate.

Specificity

This rabbit polyclonal antibody detects Prickle-like protein 1 in murine species.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~94 kDa observed; 94.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationhuman ... PRICKLE1(144165)
isotypeIgG
NCBI accession no.NP_001028389
Quality Level100
shipped inambient
species reactivity (predicted by homology)rat (based on 100% sequence homology), human (based on 100% sequence homology)
species reactivitymouse
technique(s)western blot: suitable
UniProt accession no.Q3U5C7
This product has met the following criteria to qualify for the following awards:



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