Analysis Note

ControlMouse Brain lysates

Application

Research Sub CategoryNeurodegenerative Diseases

Anti-PRG4 Antibody is an antibody against PRG4 for use in WB & IC.

Research CategoryNeuroscience

Immunocytochemistry:
Confocal IF analysis of N1E-115 using anti-PRG4 (1:500) Rabbit pAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PRG4, Proteoglycan 4, is a human gene and is found in synovial tissue, cartilage, and liver. Small amounts are found in the lung. Isoform 1 is found in the kidney, lung, liver, heart and brain. The other Isoforms, C & D, are widely expressed. PRG4 is a lubricating glycoprotein containing chondroitin sulfate and keratan sulfate glycosaminoglycans. It is responsible for boundary lubrication at the cartilage surface. A disease associated with PRG4 is Jacobs's syndrome which is an autosomal recessive disorder.

Immunogen

Epitope: C-Teminus

KLH-conjugated followed by PRG4 corresponding to the C-terminal region

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Antigen Affinity Purified

Quality

Western Blotting

Specificity

Cat. # AB2220 recognizes PRG4.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37 kDa