Application

This Anti-PPAR-gamma Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PPAR-gamma.

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected PPAR-gamma in HUVECs, A431, HeLa, HEK293, and NIH/3T3 cells..
Western Blotting Analysis: 1 µg/mL from a representative lot detected PPAR-gamma in 10 µg of human skeletal muscle tissue lysate.

General description

Peroxisome proliferator-activated receptor gamma (UniProt P37231; also known as Nuclear receptor subfamily 1 group C member 3, PPAR-gamma, PPARgamma) is encoded by the PPARG (also known as GLM1, NR1C3) gene (Gene ID 5468) in human. The peroxisome proliferator-activated receptors (PPARs) are nuclear receptor proteins that function as transcription factors. PPARs target genes play essential roles in the regulation of cellular differentiation, development, metabolism (carbohydrate, lipid, protein), and tumorigenesis. Three types of PPARs exist, namely alpha, delta (beta), and gamma. In addition, alternative splicings produce three PPAR-gamma isoforms. All PPARs heterodimerize with retinoid X receptor (RXR) and bind peroxisome proliferator hormone response elements (PPREs; AGGTCANAGGTCA) in the promoter region of target genes. PPARγ is activated by prostaglandin PGJ2 and certain members of the 5-HETE family of arachidonic acid metabolites, including 5-oxo-15(S)-HETE and 5-oxo-ETE.

Immunogen

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human PPAR-gamma.

Linkage

Replaces: 07-466

Other Notes

Concentration: Please refer to lot specific datasheet.

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PPAR-gamma in 10 µg of MCF-7 cell lysate.

Specificity

This polyclonal antibody targets an internal sequence present in all spliced isoforms of human and murine PPAR-gamma reported by UniProt (P37231 and P37238).

Target description

~55/60 kDa observed. 54.68 kDa (human PPARgamma1(wt); isoform 1), 57.62 kDa (human isoform 2), 21.58 kDa (human PPARgamma1(tr); isoform 3), 54.51 kDa (mouse isoform 1), 57.60 kDa (mouse isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.