Application

Research CategorySignaling

Research Sub CategorySignaling Neuroscience

Immunocytochemistry Analysis: 2.0 ug/mL from a representative lot detected PHLPP2 in HUVEC cells.

Anti-PHLPP2 Antibody is an antibody against PHLPP2 for use in Western Blotting and Immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PH Domain And Leucine Rich Repeat Protein Phosphatase 2 is encoded by the PHLPP2 gene. It is known to catalyze the dephosphorylation of pSer473 of AKT1 and pSer660 of PRKCbeta-II, as well as pSer657 of PRKCalpha.

Immunogen

KLH-conjugated linear peptide corresponding within the PP2C-like domain of human PHLPP2.

Epitope: Within PP2C-like Domain

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected PHLPP2 in 10 µg of HUVEC cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~150 kDa observed. Predicted to cross-react to all isoforms. Isoform 2 (~107kDa) can possibly be detected at longer exposures/higher concentrations or in certain tissues.