Analysis Note

ControlMouse and rat brain lysates

Application

Anti-PAX6 Antibody detects level of PAX6 & has been published & validated for use in WB & IC.

Immunocytochemistry:
Working dilutions of 1:50 - 1:1,000 of Anti-Pax6 was used to resolve the protein in chicken spinal cord.

General description

PAX6 is the most researched of the PAX genes and appears throughout the literature as a "master control" gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. This transcription factor is most famous for its use in the interspecifically induced expression of ectopic eyes and is of medical importance because heterozygous mutants produce a wide spectrum of ocular defects such as Aniridia in humans. This gene encodes paired box gene 6, one of many human homologues of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause aniridia as well as Peter′s anomaly, both ocular diseases.

Immunogen

KLH conjugated followed by PAX6 corresponding to the C-Terminus

Linkage

Replaces: AB5409

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Immunoaffinity purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Quality

Routinely evaluated by Western Blot on rat and mouse brain lysates.

Western Blot Analysis:
0.5 µg/mL of this lot detected PAX6 on 10 µg of mouse and rat brain lysates.

Specificity

This antibody recognizes the C-Terminus of PAX6.

Target description

~48 kDa